5VRO

ABA-mimicking ligand AMF1beta in complex with ABA receptor PYL2 and PP2C HAB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-02
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.2544
Spacegroup name	P 21 21 21
Unit cell lengths	61.294, 67.940, 145.899
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.546 - 2.257
R-factor	0.2105
Rwork	0.208
R-free	0.24420
RMSD bond length	0.004
RMSD bond angle	1.009
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.260
Rmerge	0.076
Number of reflections	29406
<I/σ(I)>	73.1
Completeness [%]	99.8
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2M di-Sodium malonate, 20% PEG3350