5VR0
Crystal structure of glucose isomerase from Streptomyces rubiginosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-01 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9792, 1.5406 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.854, 98.061, 102.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.900 - 1.700 |
| R-factor | 0.11988 |
| Rwork | 0.119 |
| R-free | 0.14161 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1xib |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.333 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.010 |
| High resolution limit [Å] | 1.700 |
| Rpim | 0.013 |
| Number of reflections | 49609 |
| <I/σ(I)> | 125 |
| Completeness [%] | 96.2 |
| Redundancy | 43.1 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M CaCl2, 16-22% MPD (v/v) and 0.1 M Tris-HCl pH 7.0 |
