5VQI

Nuclear transport of the Neurospora crassa NIT2 transcription factor is mediated by Importin-alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-30
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.45
Spacegroup name	P 21 21 21
Unit cell lengths	45.034, 63.706, 183.635
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.909 - 2.501
R-factor	0.1828
Rwork	0.179
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rxh
RMSD bond length	0.008
RMSD bond angle	1.196
Data reduction software	HKL-2000
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.910
High resolution limit [Å]	2.500
Rmerge	0.263
Number of reflections	18973
<I/σ(I)>	5.1
Completeness [%]	99.7
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.2 mM Bicine pH 8.5 and 20% (w/v) polyethylene glycol 6000