5VQH
Crystal structure of the extended Tudor domain from BmPAPI in complex with sDMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-25 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.030, 66.030, 224.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.610 - 2.400 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vqg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.482 |
| Data reduction software | iMOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.610 | 2.510 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.077 | 0.535 |
| Number of reflections | 18600 | |
| <I/σ(I)> | 8.7 | 1.6 |
| Completeness [%] | 92.8 | 95 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | 0.95 M ammonium sulfate, 50 mM sodium acetate, pH 5.5 and 5% glucose. |
