5VQD

Beta-glucoside phosphorylase BglX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-06-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	80.520, 84.150, 161.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.127 - 2.100
R-factor	0.208
Rwork	0.204
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3BMX (Sculptor edited)
RMSD bond length	0.002
RMSD bond angle	0.565
Data reduction software	HKL-3000 (HKL)
Data scaling software	Aimless (CCP4 7.0)
Phasing software	PHENIX ((dev_2481: ???))
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.720	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.095	0.259
Number of reflections	30635	2647
<I/σ(I)>	18.6	8.6
Completeness [%]	94.6	99.9
Redundancy	10.1	10.3
CC(1/2)	0.997	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	27% PEG 1000 and 100 mM MES (pH 6.5)