5VQ5

Crystal Structure of the Lectin Domain From the F17-like Adhesin, UclD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-05-16
Detector	RDI CMOS_8M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	39.010, 58.600, 175.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.570 - 1.600
R-factor	0.1739
Rwork	0.172
R-free	0.21810
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.582
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.570	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.053	0.430
Number of reflections	108142
<I/σ(I)>	17.68	1.43
Completeness [%]	99.9	99.98
Redundancy	2	2
CC(1/2)		0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 3350, 0.1 M KH2PO4, 0.2 M KI