5VPT
2009 H1N1 PA Endonuclease in complex with RO-7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-26 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 89.881, 89.881, 133.731 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.495 - 2.100 |
R-factor | 0.2012 |
Rwork | 0.200 |
R-free | 0.22030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vp8 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.495 | 2.166 |
High resolution limit [Å] | 2.091 | 2.091 |
Rmerge | 0.047 | 0.602 |
Rmeas | 0.050 | 0.658 |
Rpim | 0.017 | 0.256 |
Total number of observations | 127165 | |
Number of reflections | 16185 | 1321 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 97.5 | 82.04 |
Redundancy | 7.9 | 5.3 |
CC(1/2) | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 0.1 M HEPES pH 7.8, 1 M Ammonium Sulfate, 10 mM MnCl2, 10 mM MgCl2, 0.5% PVP K15 |