Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 41 |
| Unit cell lengths | 91.159, 91.159, 77.743 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.100 - 2.000 |
| R-factor | 0.18099 |
| Rwork | 0.180 |
| R-free | 0.21008 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2as8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.421 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.088 | 0.591 |
| Number of reflections | 40623 | |
| <I/σ(I)> | 34.2 | 4.2 |
| Completeness [%] | 94.1 | 100 |
| Redundancy | 6.5 | 6.7 |
| CC(1/2) | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 10.5 | 293 | PROTEIN SOLUTION: 0.5M L-ARGININE, 0.12M LI SULFATE, 0.004M EDTA, 0.1M CAPS, PH 10.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, PH 10.50 |
