5VPB

Transcription factor FosB/JunD bZIP domain in its oxidized form, type-I crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9787
Spacegroup name	C 1 2 1
Unit cell lengths	92.825, 99.261, 49.860
Unit cell angles	90.00, 109.40, 90.00

Refinement procedure

Resolution	43.738 - 2.691
R-factor	0.2415
Rwork	0.238
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vpa
RMSD bond length	0.002
RMSD bond angle	0.419
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.740	43.740	2.750
High resolution limit [Å]	2.691	7.320	2.691
Rmerge	0.064	0.037	0.641
Rmeas	0.074	0.044	0.748
Rpim	0.038	0.023	0.384
Number of reflections	11872
<I/σ(I)>	20		2
Completeness [%]	99.9	97.7	100
Redundancy	3.8	3.6	3.8
CC(1/2)		0.997	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	45% (v/v) 2-methyl-2,4-pentanediol (MPD), 0.3 M NaCl and 0.1 M HEPES pH 7.5