5VOO
Methionine synthase folate-binding domain with methyltetrahydrofolate from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 188.463, 188.463, 247.137 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 163.210 - 2.400 |
R-factor | 0.19235 |
Rwork | 0.190 |
R-free | 0.22972 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5von |
RMSD bond length | 0.009 |
RMSD bond angle | 1.363 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 163.210 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.092 | 0.645 |
Rpim | 0.048 | 0.342 |
Number of reflections | 100615 | 4857 |
<I/σ(I)> | 8.93 | 1.92 |
Completeness [%] | 99.4 | 98.4 |
Redundancy | 4.5 | 4.4 |
CC(1/2) | 0.996 | 0.651 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1 M sodium citrate pH 4.5, 22 % polyethylene glycol 3350, 0.2 M tri-sodium citrate, soaked with 6S-methyltetrahydrofolate |