5VOK

Crystal structure of the C7orf59-HBXIP dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	C 1 2 1
Unit cell lengths	180.884, 64.763, 79.231
Unit cell angles	90.00, 115.18, 90.00

Refinement procedure

Resolution	81.846 - 2.890
R-factor	0.2854
Rwork	0.280
R-free	0.31240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3msh 3ms6
RMSD bond length	0.008
RMSD bond angle	1.395
Data reduction software	DIALS (1.2)
Data scaling software	Aimless (0.5.31)
Phasing software	MrBUMP (00.9.00)
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.846	3.070
High resolution limit [Å]	2.890	2.890
Rmerge	0.171	3.500
Number of reflections	18719	3004
<I/σ(I)>	6.5
Completeness [%]	99.5	99.1
Redundancy	6.7	6.9
CC(1/2)	0.993	0.337

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	4M sodium formate, 5%(v/v) glycerol and 0.01M barium chloride (as additive).