5VNQ

Neutron crystallographic structure of perdeuterated T4 lysozyme cysteine-free pseudo-wild type at cryogenic temperature

Experimental procedure

Experimental method	LAUE
Source type	NUCLEAR REACTOR
Source details	ORNL High Flux Isotope Reactor BEAMLINE CG4D
Synchrotron site	ORNL High Flux Isotope Reactor
Beamline	CG4D
Temperature [K]	80
Detector technology	IMAGE PLATE
Collection date	2016-10-04
Detector	FUJI
Wavelength(s)	2.8 - 4.6
Spacegroup name	P 32 2 1
Unit cell lengths	61.500, 61.500, 95.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.700 - 2.200
R-factor	0.2431
Rwork	0.242
R-free	0.28020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lw9
RMSD bond length	0.004
RMSD bond angle	0.658
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.700	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.162	0.214
Number of reflections	7965
<I/σ(I)>	6.5
Completeness [%]	72.7
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	~2.0 M Na/K phosphate, pH 6-7, 250 mM NaCl, 40mM 2-hydroxyethyl disulfide