5VNI

Crystal structure of Sec23a/Sec24a/Sec22 complexed with a C-terminal FA sorting motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-07-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	147.606, 96.065, 129.998
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	48.803 - 2.788
R-factor	0.2147
Rwork	0.212
R-free	0.26970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nut
RMSD bond length	0.002
RMSD bond angle	0.481
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.803	2.900
High resolution limit [Å]	2.788	2.788
Rmerge	0.116	0.582
Rpim	0.083	0.427
Number of reflections	40031	3587
<I/σ(I)>	12.6	1
Completeness [%]	98.2	98.2
Redundancy	2.5	2.5
CC(1/2)		0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50mM HEPES PH 7.8, 0.5M NaAc and 12% PEG4K