5VNF

Crystal structure of Sec23a/Sec24a/Sec22 complexed with a C-terminal VV sorting motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-07-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	147.824, 97.077, 129.820
Unit cell angles	90.00, 90.12, 90.00

Refinement procedure

Resolution	48.822 - 2.407
R-factor	0.2055
Rwork	0.204
R-free	0.24120
RMSD bond length	0.003
RMSD bond angle	0.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.822
High resolution limit [Å]	2.400
Number of reflections	66635
<I/σ(I)>	1.5
Completeness [%]	99.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50mM HEPES PH 7.8, 0.5M NaAc and 12% PEG4K