5VNB
YEATS in complex with histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-08-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.790, 80.305, 121.663 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.670 - 2.400 |
| R-factor | 0.18916 |
| Rwork | 0.186 |
| R-free | 0.24771 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vna |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.643 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 27276 | 1389 |
| <I/σ(I)> | 28 | 3 |
| Completeness [%] | 97.5 | 99.9 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.4 | 290 | 1.36 M ammonium sulfate, 100 mM CHES pH 9.4 |
