5VMV

Kaiso (ZBTB33) zinc finger DNA binding domain in complex with its double CpG-methylated DNA consensus binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 2
Unit cell lengths	44.219, 183.792, 104.566
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.443 - 2.313
R-factor	0.2075
Rwork	0.203
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6n
RMSD bond length	0.011
RMSD bond angle	1.203
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.443	2.396
High resolution limit [Å]	2.313	2.313
Rmerge	0.086	0.277
Number of reflections	17383	946
<I/σ(I)>	13.11	4.29
Completeness [%]	90.8	51.16
Redundancy	6	3.6
CC(1/2)	0.996	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1 M Tris pH 8.5, 0.2 M Ammonium Acetate, 30 % (v/v) 2-propanol