5VMJ
Influenza hemagglutinin H1 mutant DH1E in complex with 3'SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-11-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.940, 81.050, 120.950 |
| Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
| Resolution | 54.825 - 2.950 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wrg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.888 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 55.380 |
| High resolution limit [Å] | 2.950 |
| Number of reflections | 39386 |
| <I/σ(I)> | 6.9 |
| Completeness [%] | 100.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.20 M NaF, 18% PEG3350 |
