5VME
Crystal structure of human IgG1 Fc K248E, T437R mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 115.010, 115.010, 73.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.511 - 3.100 |
| R-factor | 0.2312 |
| Rwork | 0.229 |
| R-free | 0.28510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6x |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.404 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (dev_1428) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.511 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.068 | 0.782 |
| Number of reflections | 10183 | 724 |
| <I/σ(I)> | 24.46 | 3.21 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.05 | 10.66 |
| CC(1/2) | 0.999 | 0.907 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 35.5% PEG 300, 0.1 M HEPES, pH 7.0 |
