5VMD

Crystal structure of UBR-box from UBR6 in a domain-swapping conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-23
Detector	RAYONIX MX300HE
Wavelength(s)	1.2822
Spacegroup name	P 21 21 21
Unit cell lengths	67.570, 69.200, 82.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.720 - 2.202
R-factor	0.1974
Rwork	0.195
R-free	0.24740
RMSD bond length	0.005
RMSD bond angle	0.684
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.720	2.281
High resolution limit [Å]	2.200	2.202
Rmeas	0.094	0.608
Number of reflections	20183	1952
<I/σ(I)>	14.67	2.88
Completeness [%]	99.8	99
Redundancy	4.3	4.1
CC(1/2)	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.2 M sodium nitrate, 0.1 M Bis-Tris propane, pH 6.5, 25% PEG3350