5VLH

Short PCSK9 delta-P' complex with peptide Pep1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	110
Detector technology	CCD
Collection date	2015-12-02
Detector	RAYONIX MX325HE
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	70.748, 70.748, 158.784
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.080 - 2.860
R-factor	0.202
Rwork	0.200
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nmx
RMSD bond length	0.008
RMSD bond angle	1.030
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.080	2.970
High resolution limit [Å]	2.860	2.860
Number of reflections	10763
<I/σ(I)>	10.7	2.2
Completeness [%]	97.2	84.7
Redundancy	7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	292	calcium acetate, PEG 6000