5VKZ

Crystal structure of Mdm12 and combinatorial reconstitution of Mdm12/Mmm1 ERMES complexes for structural studies

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	83
Detector technology	PIXEL
Collection date	2011-04-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	116.020, 116.020, 161.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	85.340 - 4.100
R-factor	0.24308
Rwork	0.242
R-free	0.25379
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gyd
RMSD bond length	0.011
RMSD bond angle	1.531
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.300	4.210
High resolution limit [Å]	4.100	4.100
Rmerge	0.115	1.050
Number of reflections	10272	744
<I/σ(I)>	9.4	2
Completeness [%]	99.5	99.7
Redundancy	7.4	7.5
CC(1/2)		0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	15-25% PEG 3350, 400 mM ammonium phosphate, 3.5 mM Mega-10