5VKL

SPT6 tSH2-RPB1 1476-1500 pS1493

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-11-16
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	43.014, 103.540, 115.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.644 - 2.198
R-factor	0.1835
Rwork	0.177
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3psj
RMSD bond length	0.007
RMSD bond angle	0.878
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.280
High resolution limit [Å]	2.198	4.740	2.200
Rmerge	0.055	0.035	0.445
Rmeas	0.056	0.035	0.458
Rpim	0.011	0.006	0.106
Number of reflections	12637
<I/σ(I)>	23.8
Completeness [%]	93.0	99.4	84.7
Redundancy	24.7	30.9	17
CC(1/2)		1.000	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	40% ethanol, 100 mM Tris pH 7.0