5VJV

Rhizobiales-like phosphatase 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-30
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.03318
Spacegroup name	P 61
Unit cell lengths	102.600, 102.600, 61.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.950
R-factor	0.1856
Rwork	0.184
R-free	0.21910
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.205
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.109	1.150
Number of reflections	25380	1958
<I/σ(I)>	12.1	1.6
Completeness [%]	99.5	98.7
Redundancy	5.5	5.5
CC(1/2)	0.998	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM Bis-Tris-Cl, 250 g/L PEG 3350, 200 g/L glycerol