5VJK
Crystal structure of H7 hemagglutinin mutant (V186K, K193T, G228S) from the influenza virus A/Shanghai/2/2013 (H7N9)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033 |
Spacegroup name | H 3 2 |
Unit cell lengths | 115.440, 115.440, 294.871 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.772 - 2.593 |
R-factor | 0.2054 |
Rwork | 0.203 |
R-free | 0.25070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n5j |
RMSD bond length | 0.012 |
RMSD bond angle | 1.666 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.593 | 2.593 |
Rpim | 0.050 | 0.440 |
Number of reflections | 23692 | 1102 |
<I/σ(I)> | 16.4 | |
Completeness [%] | 99.3 | 93.4 |
Redundancy | 4 | 2.8 |
CC(1/2) | 0.998 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.2 M tri-potassium citrate, 5% (v/v) ethylene glycol and 22% (w/v) PEG3350 |