5VJK

Crystal structure of H7 hemagglutinin mutant (V186K, K193T, G228S) from the influenza virus A/Shanghai/2/2013 (H7N9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	H 3 2
Unit cell lengths	115.440, 115.440, 294.871
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.772 - 2.593
R-factor	0.2054
Rwork	0.203
R-free	0.25070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n5j
RMSD bond length	0.012
RMSD bond angle	1.666
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.593	2.593
Rpim	0.050	0.440
Number of reflections	23692	1102
<I/σ(I)>	16.4
Completeness [%]	99.3	93.4
Redundancy	4	2.8
CC(1/2)	0.998	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	0.2 M tri-potassium citrate, 5% (v/v) ethylene glycol and 22% (w/v) PEG3350