5VJJ

Crystal structure of the flax-rust effector AvrP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-03
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.282, 1.262
Spacegroup name	P 43 21 2
Unit cell lengths	88.937, 88.937, 45.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.010 - 2.520
R-factor	0.2235
Rwork	0.222
R-free	0.26420
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.761
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	CRANK
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.010	2.650
High resolution limit [Å]	2.520	2.520
Rmeas	0.182	0.891
Rpim	0.049
Number of reflections	6602
<I/σ(I)>	16.6	2.5
Completeness [%]	99.8
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M Bis-Tris pH 7.0, 22% (w/v) PEG 3350 and 0.2 M NaCl