5VIM
Crystal structure of the Zika virus NS5 methyltransferase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.157, 109.735, 55.387 |
| Unit cell angles | 90.00, 92.57, 90.00 |
Refinement procedure
| Resolution | 30.514 - 2.100 |
| R-factor | 0.1747 |
| Rwork | 0.173 |
| R-free | 0.21610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kqr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.712 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.510 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 27143 |
| <I/σ(I)> | 4.25 |
| Completeness [%] | 97.1 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | 0.2M Lithium Sulfate, 0.1M Cacodylate, 30% PEG 400 |
