5VI8

Structure of a mycobacterium smegmatis transcription initiation complex with an upstream-fork promoter fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97919
Spacegroup name	P 1 21 1
Unit cell lengths	133.012, 161.633, 139.211
Unit cell angles	90.00, 107.72, 90.00

Refinement procedure

Resolution	51.990 - 2.760
R-factor	0.2409
Rwork	0.240
R-free	0.28090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xlp
RMSD bond length	0.002
RMSD bond angle	0.636
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	51.990
High resolution limit [Å]	2.760
Rmerge	0.234
Rpim	0.059
Number of reflections	143776
<I/σ(I)>	22.39
Completeness [%]	99.0
Redundancy	16.2
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.1 M Bis-Tris, pH 6, 0.2 M Lithium sulfate, 16% (w/v) polyethylene glycol 3350, 2.5% (v/v) ethylene glycol