5VI5

Structure of Mycobacterium smegmatis transcription initiation complex with a full transcription bubble

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97919
Spacegroup name	P 1 21 1
Unit cell lengths	132.062, 163.555, 139.964
Unit cell angles	90.00, 107.90, 90.00

Refinement procedure

Resolution	50.015 - 3.196
R-factor	0.254
Rwork	0.253
R-free	0.27990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tw1
RMSD bond length	0.002
RMSD bond angle	0.448
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.015
High resolution limit [Å]	3.196
Rmerge	0.220
Rpim	0.141
Number of reflections	88065
<I/σ(I)>	7.48
Completeness [%]	95.1
Redundancy	3.3
CC(1/2)	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.1 M Bis-Tris, pH 6.0, 0.2 M Lithium sulfate, 16% (w/v) polyethylene glycol 3350, 2.5% (v/v) ethylene glycol