5VH2

Crystal Structure of Mouse Cadherin-23 EC12-13 with Engineered Mutation S1339D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-14
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.542
Spacegroup name	P 1
Unit cell lengths	43.738, 47.210, 113.044
Unit cell angles	89.95, 90.02, 115.76

Refinement procedure

Resolution	33.990 - 2.840
R-factor	0.24
Rwork	0.238
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2whv 5tfk
RMSD bond length	0.011
RMSD bond angle	1.475
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.890
High resolution limit [Å]	2.840	2.840
Rmerge	0.165	0.377
Number of reflections	19387
<I/σ(I)>	4.3269	2.05
Completeness [%]	93.9	89.3
Redundancy	1.6	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.05 M Tris pH 8.5 0.5 M NaCl 22% PEG 4000