5VGW
RNA hairpin structure containing tetraloop/receptor motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2015-10-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 71.055, 71.055, 69.781 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.150 - 2.420 |
R-factor | 0.20764 |
Rwork | 0.207 |
R-free | 0.21293 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.755 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.073 | 0.639 |
Number of reflections | 4799 | 508 |
<I/σ(I)> | 15 | 2.33 |
Completeness [%] | 96.1 | 98.8 |
Redundancy | 3.7 | 3.1 |
CC(1/2) | 0.914 | 0.379 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 50 mM MgCl2, 0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 7.0, 25% w/v Polyethylene glycol 3,350 |