5VGT

X-ray structure of bacteriophage Sf6 tail adaptor protein gp7

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 41 21 2
Unit cell lengths	101.297, 101.297, 76.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.945 - 1.776
R-factor	0.197
Rwork	0.195
R-free	0.23350
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.550	3.340	1.810
High resolution limit [Å]	1.776	3.120	1.780
Rmerge	0.057	0.867	0.679
Rmeas	0.062	0.941	0.790
Rpim	0.024	0.361	0.395
Number of reflections	38487	1332	1936
<I/σ(I)>	17.5
Completeness [%]	98.9	99.8	89.2
Redundancy	6.1	6.7	3.6
CC(1/2)	0.999	0.770	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	PEG 8000, MES 6.0, calcium acetate