5VGE
Crystal structure of HLA-C*07:02 in complex with RYR peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.954 |
Spacegroup name | P 65 |
Unit cell lengths | 99.670, 99.670, 77.914 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.160 - 2.600 |
R-factor | 0.16629 |
Rwork | 0.163 |
R-free | 0.22082 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nt6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.384 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.160 |
High resolution limit [Å] | 2.600 |
Rpim | 0.050 |
Number of reflections | 13655 |
<I/σ(I)> | 14.8 |
Completeness [%] | 100.0 |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M HEPES pH 8.5 2 mM ZnSO4 28 % jeffamine ED-2001 (Hampton) |