5VGD

Crystal Structure of HLA-C*0501 in complex with SAE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-28
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	44.062, 80.538, 118.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.740 - 2.320
R-factor	0.2243
Rwork	0.220
R-free	0.29720
RMSD bond length	0.010
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.740	2.390
High resolution limit [Å]	2.320	2.300
Rpim	0.057	0.387
Number of reflections	19238	1744
<I/σ(I)>	11.5	2.1
Completeness [%]	99.4
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	1.8M NaMalonate pH7