5VFY

Structure of an accessory protein of the pCW3 relaxosome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953700
Spacegroup name	P 21 21 2
Unit cell lengths	80.560, 120.590, 52.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.210 - 2.490
R-factor	0.184
Rwork	0.181
R-free	0.23300
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	Aimless (0.3.11)
Phasing software	SHELXDE
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.440	48.440	2.590
High resolution limit [Å]	2.490	8.970	2.490
Rmerge	0.214	0.091	0.869
Rmeas	0.232	0.101	0.951
Rpim	0.088	0.043	0.373
Number of reflections	18698
<I/σ(I)>	10.8
Completeness [%]	99.5	93.6	97.2
Redundancy	6.3	5.3	5.9
CC(1/2)	0.984	0.986	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1 M Na Citrate, 0.1 M Na Cacodylate, 0.1M Phenol