5VFA
RitR Mutant - C128D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.836, 59.708, 52.509 |
| Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
| Resolution | 27.519 - 1.452 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.18370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u8k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.954 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2450: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.046 | 0.498 |
| Number of reflections | 76689 | 3384 |
| <I/σ(I)> | 28.6 | 2.9 |
| Completeness [%] | 99.0 | 89.1 |
| Redundancy | 5.2 | 4.3 |
| CC(1/2) | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1 M HEPES pH 7.5, 22.5% Jeffamine ED-2001 pH 7.0 |
