5VEX
Structure of the human GLP-1 receptor complex with NNC0640
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2016-11-30 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.810, 67.510, 83.660 |
| Unit cell angles | 91.55, 89.88, 107.56 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 5ee7 and 212L |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 0.600 |
| Number of reflections | 24745 | |
| <I/σ(I)> | 5 | |
| Completeness [%] | 91.4 | 87.7 |
| Redundancy | 2.9 | 2.3 |
| CC(1/2) | 0.990 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.4-0.45 M ammonium acetate, 0.1 M sodium acetate, pH 5.0-5.8, 38-40% PEG400 |
