5VEB
Crystal structure of a Fab binding to extracellular domain 5 of Cadherin-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.829, 88.855, 186.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.230 - 2.340 |
| R-factor | 0.2144 |
| Rwork | 0.212 |
| R-free | 0.25230 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.230 | 2.348 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.083 | 0.595 |
| Number of reflections | 55887 | 535 |
| <I/σ(I)> | 17.3 | 3.1 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 20% (w/v) PEG3350, 0.2M di-ammonium hydrogen citrate |
