5VE9

Structure of hACF7 EF1-EF2-GAR domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.28295
Spacegroup name	P 31 2 1
Unit cell lengths	92.984, 92.984, 90.446
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.349 - 2.795
R-factor	0.258
Rwork	0.256
R-free	0.27880
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.750
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.130	0.068	1.156
Rmeas	0.134	0.070	1.188
Rpim	0.029	0.016	0.267
Total number of observations	244650
Number of reflections	11525
<I/σ(I)>	6.9
Completeness [%]	100.0	99.8	99.9
Redundancy	21.2	20.1	18.7
CC(1/2)		0.999	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	potassium chloride, PEG3350