5VDR
Human cyclic GMP-AMP synthase (cGAS) in complex with 3',3'-cdIMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2016-07-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 215.528, 48.395, 89.099 |
| Unit cell angles | 90.00, 109.89, 90.00 |
Refinement procedure
| Resolution | 55.901 - 3.042 |
| R-factor | 0.2104 |
| Rwork | 0.208 |
| R-free | 0.25350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o67 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.604 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | PHENIX (dev_1999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.901 | |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.101 | 0.491 |
| Rpim | 0.095 | 0.463 |
| Number of reflections | 16476 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 96.9 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 18-20% PEG 3350, 0.2 M ammonium citrate pH 7 |
