5VDF
Crystal Structure of Cu(I)-loaded yeast Atx1: Crystal Form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.148, 114.385, 58.135 |
| Unit cell angles | 90.00, 92.32, 90.00 |
Refinement procedure
| Resolution | 58.090 - 1.930 |
| R-factor | 0.19782 |
| Rwork | 0.195 |
| R-free | 0.24175 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cc8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.394 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.090 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.087 | 0.751 |
| Rpim | 0.075 | 0.669 |
| Number of reflections | 45429 | 2967 |
| <I/σ(I)> | 9.8 | 1.4 |
| Completeness [%] | 99.5 | 94.6 |
| Redundancy | 4.3 | 3.9 |
| CC(1/2) | 0.998 | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 0.1 M HEPES (pH 7.9), 20% (w/v) PEG3350 |
