5VBN
Crystal Structure of human DNA polymerase epsilon B-subunit in complex with C-terminal domain of catalytic subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-17 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.260, 201.459, 78.336 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.750 - 2.350 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.26400 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.072 | 0.481 |
Number of reflections | 61804 | 3044 |
<I/σ(I)> | 21.5 | 2.2 |
Completeness [%] | 98.6 | 98.2 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 50 mM sodium citrate, pH5.6, 0.70 M ammonium sulfate and 2 mM TCEP |