5VBC
Crystal structure of ATXR5 in complex with histone H3.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.170, 87.310, 74.510 |
| Unit cell angles | 90.00, 127.78, 90.00 |
Refinement procedure
| Resolution | 26.404 - 2.100 |
| R-factor | 0.1981 |
| Rwork | 0.196 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o30 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.997 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 29410 |
| <I/σ(I)> | 12.1 |
| Completeness [%] | 98.3 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 50% polypropylene glycol 400, 100mM Na-Hepes pH 6.0 and 5% DMSO |
