5VB9

IL-17A in complex with peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-29
Detector	MARRESEARCH
Wavelength(s)	0.97931
Spacegroup name	P 21 21 2
Unit cell lengths	36.771, 55.122, 143.953
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.470 - 1.700
R-factor	0.1834
Rwork	0.182
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.470	71.977	1.790
High resolution limit [Å]	1.700	5.380	1.700
Rmerge		0.057	0.512
Rmeas	0.094	0.067	0.608
Rpim	0.036	0.026	0.238
Total number of observations	220360	7093	29556
Number of reflections	32769
<I/σ(I)>	12.6	24.7	3
Completeness [%]	99.1	96.4	98.3
Redundancy	6.7	6.2	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	281	Protein at 7.1 mg/ml in 10mM Bis-Tris pH 6.5, 10% glycerol, 150mM NaCl with 2 mM of peptide, equilibrated against a reservoir containing 20% PEG 3350 and 200mM Lithium Chloride.