5VAQ

Crystal Structure of Beta-Klotho in Complex with FGF21CT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	48.647, 145.487, 213.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.007 - 2.606
R-factor	0.193
Rwork	0.191
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.287
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	64.007
High resolution limit [Å]	2.606
Rmerge	0.090
Number of reflections	46776
<I/σ(I)>	13.12
Completeness [%]	98.0
Redundancy	3.9
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	293	14% PEG 4000, 0.1M MES