5VA7

Glucocorticoid Receptor DNA Binding Domain - IL11 AP-1 recognition element Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	39.143, 96.855, 104.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.793 - 2.153
R-factor	0.2027
Rwork	0.201
R-free	0.22700
RMSD bond length	0.009
RMSD bond angle	1.058
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.260
High resolution limit [Å]	2.180	4.700	2.180
Rmerge	0.085	0.064	0.575
Rmeas	0.094	0.072	0.672
Rpim	0.040	0.032	0.340
Total number of observations	117427
Number of reflections	21954
<I/σ(I)>	10.6
Completeness [%]	99.6	99.5	96.4
Redundancy	5.3	4.9	3.5
CC(1/2)		0.993	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Lithium Nitratie, 15 % PEG 3350, 1 % glycerol