5VA4
TRIM-Cyclophilin A B-box 2 and coiled-coil chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2017-02-05 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 104.596, 104.596, 104.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.954 - 2.306 |
| R-factor | 0.2364 |
| Rwork | 0.231 |
| R-free | 0.28740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eiu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.375 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.045 | 0.681 |
| Number of reflections | 8544 | 1281 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 10.6 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 17% PEG 3350, 0.4 M sodium/potassium tartrate |
