5V97
Structure of the H477R variant of rat cytosolic PEPCK in complex with GTP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-07-30 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.281, 118.983, 60.974 |
Unit cell angles | 90.00, 106.87, 90.00 |
Refinement procedure
Resolution | 32.800 - 1.800 |
R-factor | 0.2139 |
Rwork | 0.212 |
R-free | 0.24430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qey |
RMSD bond length | 0.007 |
RMSD bond angle | 1.252 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.080 | 0.048 | 0.607 |
Total number of observations | 303532 | ||
Number of reflections | 57690 | ||
<I/σ(I)> | 20 | ||
Completeness [%] | 99.1 | 98.6 | 94.2 |
Redundancy | 5.3 | 6.1 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 24 - 34% PEG 3350 and 100mM HEPES, at pH 7.4 |