5V7U

Cyrstal structure of anti-Tau antibody CBTAU-22.1 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	52.467, 67.229, 62.470
Unit cell angles	90.00, 94.40, 90.00

Refinement procedure

Resolution	45.691 - 1.640
R-factor	0.1781
Rwork	0.176
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lri 4jpk
RMSD bond length	0.007
RMSD bond angle	1.208
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.670
High resolution limit [Å]	1.640	1.640
Rpim	0.050	0.410
Number of reflections	53078
<I/σ(I)>	23.7
Completeness [%]	99.9	100
Redundancy	3.7	3.5
CC(1/2)	0.995	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	295	0.1 M citrate buffer, pH 4.0, 2.4 M ammonium sulfate