5V7O
Crystal Structure of NosK from Streptomyces actuosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.290, 75.290, 110.121 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.053 - 2.300 |
R-factor | 0.2232 |
Rwork | 0.219 |
R-free | 0.26270 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.863 |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.053 | 28.050 | 2.380 |
High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
Rmerge | 0.170 | 0.044 | 1.155 |
Number of reflections | 16566 | ||
<I/σ(I)> | 12.7 | ||
Completeness [%] | 99.9 | 97.1 | 100 |
Redundancy | 10.8 | 9 | 11.1 |
CC(1/2) | 0.980 | 0.972 | 0.921 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.6 M (NH4)2SO4, 0.1 M NaCl, 0.1 M HEPES, pH 7.5 |