5V7B
Crystal structure of Influenza A virus matrix protein M1 (NLS-88E)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-03-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.190, 96.170, 48.490 |
| Unit cell angles | 90.00, 100.86, 90.00 |
Refinement procedure
| Resolution | 27.580 - 2.500 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ea3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.910 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 | 0.379 |
| Number of reflections | 12404 | 1227 |
| <I/σ(I)> | 9.5 | 2.7 |
| Completeness [%] | 98.7 | 99.3 |
| Redundancy | 3.57 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 277 | 16.7 mg/ml protein in 25mM Hepes/NaOH, 0.2M NaCl, 2mM TCEP, pH 7.0 |
